Laserchrom HPLC Laboratories Ltd
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Laserchrom HPLC Laboratories Ltd

Units B16-18, Laser Quay,

Medway City Estate,

Rochester, Kent. ME2 4HU (United Kingdom)

Useful HPLC Information

This section contains a little background information useful to HPLC. To access the information, click on the section heading below:

This section covers the Resolution Equation, and how to measure it from a chromatogram using the selectivity, relative retention time and efficiency parameters. There's also a section on Solvophobic theory and Acid-Base Equilibria.
HPLC books or manuals are full of acronyms and this is a fairly comprehensive list to help you through the maze!
A guide to the solvent properties relevant to HPLC, such as viscosity, UV cut-off, refractive index, polarity, boiling point, miscibility, dipole etc. Each is explained with nrespect to HPLC and then there is a table giving these properties of the most commonly used HPLC solvents.

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