HPLC Products

If you have lunchtime meetings for your department, and would like to have one of the following presentations, Laserchrom offer this service for £100 provided that your company is within daily travelling distance of Laserchrom at Rochester, Kent.

Each presentation lasts about an hour, although this can be varied to suit specific time constraints. If a suitable projector is available, then Powerpoint may be used, although this is not a requirement.

There is no specific requirement for the meetings to be at lunchtime, although this is what is normally offered. Printed notes are not provided, but a certificate can be issued to all those participating for training records

• High temperature (up to 200oC)
• High Pressure (up to 18,000psi)
• Columns (bonding, end-capping, pH resistance, new supports)
• Advances in LC-MS
• Nano LC

Many samples contain basic groups and require high pH to suppress ionisation and obtain sharp peaks. Polymer supports solve these problems at a stroke, and since September 2006 there is no price penalty. But there are several changes to a method which have to be made.

Unravelling the mysteries of Gradient Elution

• What really happens in a column during gradient elution
• Developing a gradient profile for the first time
• The effect of changing gradient slope
• Separation of complex mixtures
• How to halve a method run time

The application of HPLC theory to achieve a good separation

There are only three variables in the resolution equation. We consider how can they be changed in real life, and what effect can we expect from each of these changes.

Overview of integration, including setting up a calibration

Optimising integration is very important for avoiding errors, and establishing confidence in results. This is a refresher with something for everyone!

This presentation presents a series of problem scenarios, and the faults are found by discussion.

eg. The pump is running, the drop rate into the waste bottle is normal, the baseline is normal, the back pressure is about 500psi higher than normal, and the peaks (but not the solvent front) are eluting late. Whats wrong?