They key to troubleshooting is to systematically eliminate possibilities, thereby homing in on the problem. From there it requires only minimal work to identify the cause.
We offer an HPLC Troubleshooting Course, where the solutions to all these problems and many more are covered. This is recommended for all those who need to be able to fix problems as they arise, and prevent problems recurring.
If you have problems with a method or an instrument, we also offer an on-site troubleshooting service. This is charged by the hour or by the day, and can include training, instrument repairs if necessary, and making a method work for you in your lab. To enquire about this service, please send an email to email@example.com, outlining the problem and what you would like to achieve, and we will call you to discuss the options.
There are six indicators that should be considered when a problem arises, namely the back pressure, the baseline, peak retention times, peak heights, the retention time of the void volume peak and the drop rate into the waste bottle. These between them will suggest the answer to most problems. To learn how, you need to come on the course!