Laserchrom HPLC Laboratories Ltd

Making HPLC work for you!

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     > Laserchrom Home Page                                                                                                                            United Kingdom

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HPLC Method Development

 

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Sometimes a new method is required. This may be for a product which has never been analysed anywhere before, or simply for an application which has not been done in your particular lab. Either way, a method must be sourced or prepared, and then it must be implemented, optimised and validated. This can be very time-consuming and without a high level of competence in HPLC, can lead to a method which works now but gives problems later.

We have quite large libraries of methods which have been done before. So the first way we may be able to help is to avoid re-inventing the wheel! If a method cannot be found, trials need to be undertaken to establish a starting point for method development and optimisation.

It is helpful to have a diode array detector or LC-MS to assist in peak identification and tracking, and it may be necessary to try a number of different columns. For some applications (eg sugars by ligand exchange, or GPC) the columns can be over £1,000 each, so this can be a very expensive process.

Laserchrom has over 1,000 columns and almost every type of detector available. So the expense of trying these options is not so great. In the main, new detectors and new columns do not need to be purchased for evaluation.

Method development can be very time-consuming. There are many columns to try, and an almost infinite combination of eluent, pH, temperature, etc to choose from. This can mean doing a very large number of analyses, and may still not find the optimum conditions. We have Drylab software, which allows us to optimise all of the above and more in the minimum of runs, and provides very accurate predictions for the otpimum conditions. So again it is most likely that we can provide not only a better method at the end, but in much less time, and for much less money. .................

At Laserchrom we have 27 different reversed phases from Dr Maisch, Germany, along with four different types of polymer-based reversed phase columns from Shodex. We can evaluate any or all of these for any method as necessary, along with many others for other applications. We have many normal phase columns, along with ion exchange, ion chromatography, ligand exchange for sugars, ion exclusion for organic acids, size exclusion, and mixed mode columns for more complex samples.

Method Optimisation will include the type of oprganic solvent, the %B, temperature, pH, buffer type, buffer concentration, column type, detection mode and detector settings (eg wavelength, voltage for ECD etc)

We are also able to identify peaks accurately, and optimise detector conditions for maximum sensitivity. Once a method is established, we can then carry out a validation exercise, before bringing the method to your lab to set up on your equipment, and we can provide on-site training to ensure that the results work for you too.

We are happy to discuss your requirements, and if some parts of the process can be done in your lab, we will be pleased to work alongside and fill in the gaps where you either don't have the time, columsn or equipment to do the work in-house. Or we could develop the method and hand it back to you for validation.

Sample pretreatment or extraction can be part of the work we do, or if you feel confident to undertake this, we can work with cleaned up samples ready for injection.

Once the method is transferred to your lab, we provide telephone technical support free of charge, and on-site support, training and troubleshooting for a daily rate.

 
 
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